Efficient gel extraction and PCR cleanup

The Gel / PCR DNA Fragments Extraction Kit is designed to recover or concentrate DNA fragments (50 bp - 10 kb) from agarose gel, PCR or other enzymatic reaction. The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and denature enzymes, and than DNA fragments in chaotropic salt are bonded to glass fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides could be effectively removed from reaction mixture without phenol extraction and alcohol precipitation. Typical recoveries are 60-80% for gel extraction and 80-90% for PCR cleanup. The entire procedure can be completed in 20 minutes and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reaction.

Fast
Operation time < 20 minutes

Safe
No phenol extraction and alcohol precipitation

Convenient
Purified DNA fragments are ready to use for PCR, sequencing, restriction digestion, DNA labeling, ligation

Stable
High recovery, low elution volume

Contents
DF Buffer
Wash Buffer (concentrated)
Elution Buffer (10mM Tris-HCl)
DF Column
2 mL Collection Tubes

Quality Control
The quality of Gel/PCR DNA Fragments Extraction Kits is tested on a lot-to-lot basis. The efficiency of DNA recovery are tested by isolation of DNA fragments of various sizes from either aqueous solution or agarose gel. The Purified DNA is checked by agarose gel analysis.

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