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Description:
DCSPol is a highly processive, thermostable DNA polymerase.
Due to its genetic modifications DCSPol has an enhanced stability
at room temperature with no activity loss for up to 1 month.
The enzyme has 5→3 polymerization-dependent exonuclease
replacement activity but lacks 3→ 5 exonuclease activity.
Source:
Purified from an E.coli strain that carries an overproducing plasmid
containing a modified gene of Thermus aquaticus DNA Polymerase.
Applications:
Suited for a wide range of PCR assays
TA cloning
Quality control:
The enzyme is free of nicking and priming activities, exonucleases
and non-specific endonucleases. SDS/PAGE - 95 kD band, >98%
pure. Activity and stability tested via thermo-cycling. The error rate
per nucleotide per cycle is ~ 2.5 x 10-5; the accuracy is ~ 4 x 104.
Estimated half life at 95ēC is 1.5 hours.
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