Description:
DCSHot DNA Polymerase is a chemically modified DCSPol
DNA Polymerase. At ambient temperatures it is inactive,
having no polymerization activity. DCSHot DNA Polymerase
is activated by a 15 min incubation step at 95°C. This
prevents extension of non-specifically annealed primers
and primer-dimers formed at low temperatures during PCR
setup. The enzyme has 5’→3’ polymerization-dependent
exonuclease replacement activity but lacks 3’→ 5’ exonuclease
activity.

Source:
Purified from an E.coli strain that carries an overproducing
plasmid containing a modified gene of Thermus aquaticus
DNA Polymerase.

Quality control:
The enzyme is free of nicking and priming activities,
exonucleases and non-specific endonucleases. SDS/PAGE
- 95 kD band, >98% pure. Activity and stability tested via
thermo cycling. The error rate per nucleotide per cycle is
~ 2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life
at 95ºC is 1.5 hours.