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GENEzol TriRNA Pure Kit (50 reactions)

157,00 78,00

The GENEzol™ TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Description

Advantages

  • Purify total RNA in 15 minutes!
  • A cost effective phenol, guanidine isothiocyanate solution plus spin column system
  • No chlorophorm phase separation
  • No isopropanol RNA precipitation
  • No phenol carryover
  • Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma, up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
  • Spin Columns: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
  • Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, other components should be stored at room temperature (15-25ºC)

Applications

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

Components

  • GENEzol™ Reagent
  • Pre-Wash Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

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