The Plant Virus RNA Kit uses a simple and efficient spin column procedure to purify virus RNA from a wide range of virus infected plant species including those with high levels of polysaccharide inhibitors. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the spin column. The procedure does not require phenol extraction or alcohol precipitation and can be completed within 25 minutes. A variety of high-quality plant viral RNA can be purified from 100 mg plant tissue samples and can be used directly in a variety of sensitive downstream applications.